This is called a Gating Tree FlowJo is an integrated environment for viewing and analysing flow cytometric data, presented in the form of a Workspace. When you create gates in a sample or subpopulation, FlowJo shows the new subsets in the Workspace in a hierarchical manner each subpopulation is shown indented under the sample or subset that it came from.
The backgating icon shows up graph window for a subset that is gated more than once (or you can choose Backgating Analysis. FlowJo's backgating analysis provides a tool to view the effect of every gate in the gating tree on the final gated population. 2) Take (gate) 3 and apply it to (gate) 1, creating a new subpopulation we'll call 3b. You tell FlowJo to backgatehere is what FlowJo does: 1) Take (gate) 3 and apply it to (gate) 2 (you'll see no change in the workspace). Isolate, divide, or group your populations for excellent annotation and analysis
This menu allows you to choose whether to. Show Child graph: This allows you to select what parameters FlowJo will use on the axes when you open the graph of a sub-population. Use Gating-ML conventions: Check this box to save a second file besides the workspace file that describes the gates in the workspace using this industry standard format.When you set a gate to select a subset of the cells, FlowJo creates a new node-i.e., a new population When you collect a sample and import it into the workspace, FlowJo creates a node to represent that set of events.
The process of gating simply creates a new population.FlowJo offers a whole set of Gating and Advanced Gating tools Subsets can be gated to generate further subsets, until a collection has only the cells for which a graphic display or analysed statistics are desired. Gating : Subsetting collected events for further analysis is critical in analysis of flow cytometry data.Home FlowJo gating Graphs and Gating FlowJo Documentation - Just another